FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070394

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070394
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12269048
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCAAT15750.033.70758433
CTCGTAT16150.032.59546742
CGTATGC16450.032.1396144
TCTCGTA16950.031.32248341
TATCTCG17650.030.0792539
GTCACGC17900.029.78445829
ACGCCAA18200.029.41722932
TCGTATG18100.029.33345643
ATCTCGT18400.028.97545440
GATCGGA18750.027.9999711
AGTCACG20750.025.80199428
ATCGGAA20950.025.2743322
ACACGTC21700.024.98300213
TCGGAAG21900.024.2805443
CGTCTGA22650.023.83583516
CGGAAGA24350.022.1140464
ACGTCTG24450.022.08104915
GTATGCC24700.021.7874445
CACGTCT25900.021.19226514
GCACACG28100.019.37297611
Produced by FastQC (version 0.11.5)