FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4874155
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2450.019.28725842
CGTATGC2700.017.5012244
TCTCGTA2800.016.07271641
GATCGGA2700.015.8367121
AACGGGA12250.015.4279068
CGTATAG1056.0320634E-415.00320151
TCGTATG3100.014.51699343
CGTTGGA14950.014.4512441
AAACGGG15350.014.3644967
ACGGGAT9100.013.8455579
CCGTCCG1000.007555221713.5001115
GAAACGG16000.013.3594846
CGGGATT8650.013.26532410
TCTCGAC1709.419873E-613.23499531
GGAAACG16850.013.2196925
ACCGCGC4500.012.99970731
ATCGGAA3650.012.9473042
CACCGCG4800.012.65583530
ATCTCGT3800.012.4349540
CGCGCCT1651.0442574E-412.27270233
Produced by FastQC (version 0.11.5)