FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5329843
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA16550.016.114651
ACGGGAT10950.013.9697869
AACGGGA14350.013.9518918
CGGGATT9850.013.7028210
AAACGGG18100.013.6712757
GGAAACG18700.013.2378435
GAAACGG19550.012.8925076
CGCCCGG6100.011.80123135
CCTACGG2301.2858764E-611.7920761
ACCGTAC1350.0040099311.66683231
ACCGCGC4250.011.64722431
CGCGCCC3602.237357E-1011.24910433
CACCGCG4509.094947E-1210.50014930
ACGGGTT3704.1909516E-910.3357549
CCCGGCC10750.010.04476937
GCGTGAG13050.09.99798422
CCCAAAG61750.09.9922321
CCCGTAA2057.4791146E-49.92261
GCCCGGC12650.09.78091136
ACAGGCG18200.09.76506518
Produced by FastQC (version 0.11.5)