FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6952422
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT13550.014.603699
AACGGGA19200.013.9368628
CGTTGGA22200.013.6504051
AAACGGG23000.013.1985187
CGGGATT14000.013.00996810
GAAACGG24150.012.7609216
CGCCCGG6000.012.36847935
ACAGGCG21450.011.845963518
GGAAACG25200.011.7333045
CCCGGTA1751.7234082E-411.6304951
ACGCCCG3358.1490725E-1011.41246834
CACCGCG5250.010.71048830
CGCGCCC4001.2369128E-1010.68386633
ACCGCGC4900.010.55763431
CGGGAAT8900.09.85357610
GCGTGAG16300.09.65762322
TACAGGC54000.09.4109617
CAGGCGT20000.09.331840519
GAGCCAC45350.09.32267226
CCCAAAG76100.09.3015091
Produced by FastQC (version 0.11.5)