FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8969200
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA24550.013.14654351
ACGGGAT15650.012.9380299
AACGGGA21400.012.6155868
AAACGGG25750.012.3195937
CGGGATT15250.012.24470110
GAAACGG25850.012.0107636
CTCGTAT3401.036824E-911.2489644
GGAAACG28600.010.8557615
CGCGCCC5200.010.81618733
CGCCCGG9850.010.73504835
TCTCGTA3755.2186806E-910.19905843
ACCGCGC6250.09.71893931
ACGCCCG5353.6379788E-129.67200434
TCGTATG4051.8389983E-89.44357245
CCCCGAT2453.7549675E-49.2121491
TATCTCG4203.3238393E-89.1062541
CCGCGCC6950.09.06380932
GCCACCG20300.08.97680328
GCGTGAG21250.08.89324722
AACGGGT4854.0781742E-98.813578
Produced by FastQC (version 0.11.5)