FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8766025
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA7750.029.89654241
CGATCAG7900.029.04447233
CTCGTAT8300.027.9154542
GTCACGA8800.026.07371929
CGTATGC9300.025.39754944
TCACGAT9200.025.1845930
GATCGGA9250.024.8526131
TCGTATG9600.024.60387843
ACGATCA9600.024.13550832
ATCTCGT10150.023.04916840
ATCGGAA10500.022.5379092
TCGGAAG11900.020.8315753
AGTCACG11250.020.59539628
ACACGTC11550.020.25521513
CGTCTGA12450.019.33302716
CACGATC13350.018.1981831
ACGTCTG13100.017.68688615
CGGAAGA14350.017.2749654
GTATGCC13950.017.1023245
CACGTCT13900.016.66893614
Produced by FastQC (version 0.11.5)