FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9216256
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT13500.012.7748179
CGTTGGA21150.012.6363391
AACGGGA17900.012.3155818
AAACGGG21600.011.872257
CGGGATT12850.011.08692510
GAAACGG23000.010.9585616
GGAAACG23050.010.9413825
GCGTGAG15550.010.0298322
CGTGAGC17050.09.58722123
ACAGGCG21850.09.05968618
ACCCGAT1500.00180922829.0087171
CCACGAT3501.9281288E-109.0087171
CCCTAAA22100.08.5603191
CCCAAAG73350.08.228821
CACTGCG6400.08.20741530
CGGGATA3305.3900294E-88.17992310
GAGCCAC45200.08.09546926
CGGGAAT7850.08.02361810
CGCTAAT1700.00560965767.9488681
AGGCGTG21550.07.933196520
Produced by FastQC (version 0.11.5)