FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11247075
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG439520.39078604881713686TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT51750.040.8255844
TCTCGTA52150.040.46857543
TCGTATG53500.039.5322245
ATCTCGT54150.039.0154442
AACGATC55800.037.8615938
AAACGAT57500.036.78035437
ACGATCT57950.036.3792439
GTCACGT59400.035.605329
AGTCACG60500.034.88355328
GAAACGA61450.034.85593436
TGAAACG61450.034.67240535
CGTGAAA61400.034.6641533
GATCGGA63600.033.7760731
ACGTGAA63150.033.7391732
CACGTGA63450.033.4744631
TCACGTG63550.033.42178730
GATCTCG63900.033.20322441
CGTCTGA65150.032.91151416
ACACGTC65600.032.71932213
ATCGGAA66050.032.6352652
Produced by FastQC (version 0.11.5)