FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6691686
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAA3000.014.267571
CGTTGGA18500.012.78601551
GCGTGAG14600.012.01954222
CGTGAGC15200.011.54508723
AAACGGG18750.011.5190377
GAAACGG19250.011.219846
AACGGGA15750.010.8562348
GGAAACG19000.010.7804885
CCCCGTT3359.638825E-910.759521
ACGGGAT11100.010.5396599
CGGGAAT7700.010.518610
ACAGGCG21850.010.39958818
CCACGAC3206.417449E-79.8558871
CCCGGAT3053.704734E-69.6019891
AACGGGT4501.0568328E-99.4992058
TATGCCG2150.00120148119.4222523
GGCGTGA19350.09.41781621
CCGGATT2701.0024026E-49.1737152
GAGCCAC47050.08.98968326
CGGGATT9850.08.90788410
Produced by FastQC (version 0.11.5)