FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12457897
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC1583191.2708324687545578TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA179000.043.4831281
CTCGTAT185000.041.54733742
TCTCGTA185450.041.41013341
ATCGGAA187950.041.3835372
TCGGAAG190000.041.067483
CGTCTGA189200.041.04115316
ACACGTC189500.041.03553413
CGTATGC187800.041.01173444
ATCTCGT187400.040.9672340
TCGTATG187850.040.9648943
CGGAAGA192450.040.849074
ACGTCTG191950.040.47661215
CATCTCG189650.040.44561439
CACGTCT193600.040.166514
GTATGCC193650.039.71019445
GCACACG197950.039.55655311
CACACGT200650.039.0354812
GATCATC206150.037.32842636
ACCAGAT209850.036.8203332
AGAGCAC214400.036.773358
Produced by FastQC (version 0.11.5)