FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5432124
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGGG16650.014.3239967
AACGGGA13750.013.2539978
CACCGCG4650.012.5802430
CGTTGGA17200.012.5615521
GAAACGG17950.012.5348786
CGGGATT10300.012.45094210
ACGGGAT10900.011.9719839
GGAAACG18750.011.5200535
CCGGCTA7050.011.48952138
CGGGATA2351.7599177E-611.48888719
AACGGGT4151.8189894E-1211.3850728
ACCGCGC4850.011.13376631
CGCGCCC3307.767085E-910.909142533
GTTCGGG1500.00878384510.500056
CGCCCGG6450.010.46516535
ACGCCCG3908.9858077E-1010.38466434
CGGCTAA7250.010.24180439
CAAGCGC2504.3415865E-59.9026911
GCCCGGC13400.09.73894236
CCCATAA16050.09.6755261
Produced by FastQC (version 0.11.5)