FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5312773
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA18550.015.6887241
AAACGGG18600.014.2726127
AACGGGA15550.014.1784938
ACGGGAT10850.014.0998199
GAAACGG19150.013.9835966
CACCGCG4850.013.91663830
CCGCGCC4650.013.547523532
GGAAACG20050.013.46838955
TCTCGTA3350.013.43185341
ACCGCGC4900.013.31547831
CTCGTAT3500.012.85620242
CGGGATT10350.012.82466510
GCGTGAG12500.012.23864422
GATCGGA3356.002665E-1112.1218671
TCGGAAG3905.456968E-1211.5405493
CGTGAGC13350.011.29109623
CGGGAAT7000.011.24875410
ATCGGAA3856.184564E-1111.1062182
ACAGGCG17850.010.96517118
CGGGATA1450.00683568210.86086610
Produced by FastQC (version 0.11.5)