FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8608958
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6300.024.3451745
GATCGGA8150.018.2832531
TCTCGTA11050.015.26513340
CTCGTAT11000.015.1296241
CGTATGC10900.015.06200643
CGGGATT17200.014.12095610
TCGTATG11950.013.92684642
ACACGTC13150.013.68147912
CATCTCG12600.013.56601238
ACGGGAT19450.013.412429
CGTTGGA29500.012.7809041
AACGGGA25700.012.6883088
ATCGGAA12750.012.3875132
AAACGGG30800.012.0476417
GAAACGG31850.012.00476556
GGAAACG31200.012.0043775
ATCTCGT13500.011.9951439
CGTCTGA14100.011.96226715
ACGTCTG14750.011.58758414
TCGGAAG13400.011.2814852
Produced by FastQC (version 0.11.5)