FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6556070
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG76510.11670101142910311TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT11500.034.03627844
TATCTCG11650.033.4049541
TCTCGTA11800.032.98031243
TCGTATG12200.031.9182245
GTCACGA12150.031.1045329
ATCTCGT13350.029.15113642
GATCGGA12950.028.8882541
AGTCACG14000.027.95837228
ACACGTC14850.026.66102613
TCGGAAG14250.026.5666983
ACGAGTG14500.026.5288732
ATCGGAA14200.026.5035762
CACGAGT15200.025.45514131
CGTCTGA15700.024.78774816
CGGAAGA15600.024.4121074
ACGTCTG16550.023.65058515
CACGTCT18050.022.05904614
GCACACG18350.021.69840611
CACACGT19600.020.54412512
GGATATC19250.020.3333638
Produced by FastQC (version 0.11.5)