FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4696457
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1701.7680577E-917.2543623
ACCGCAC3400.013.22991231
CTCGTAT2257.437484E-812.99485144
CCGCACC3501.0913936E-1112.20931832
TCTCGTA3001.7535058E-911.9951243
ACGCCCG1902.9641642E-511.83728934
CGTATGC2351.7003022E-611.5217341
TATCTCG2604.872272E-711.24554441
CGTTGGA12600.011.1026231
CGCACCT2851.3348108E-711.04813733
GCGTGAG10900.010.72969322
GCCACCG9550.010.59778228
CGTGAGC11250.010.59580223
ATAGGCG1500.00881037810.4957318
ACCGCGC2801.2662786E-610.4421831
AAACGGG12350.010.3802817
CCACCGC6950.010.35549829
GAAACGG12850.010.3274176
CGGGATT6550.010.30104310
CCCAAAG51050.010.254081
Produced by FastQC (version 0.11.5)