FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7158628
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA17950.012.9043251
ACGGGAT10850.012.8566919
AAACGGG20050.011.895017
AACGGGA16450.011.6714858
GAAACGG21000.011.215856
GGAAACG20350.011.13161855
ATAGGCG2651.10958354E-1010.75306118
CGGGATT10700.010.65271610
AACGGGT3850.010.5192578
CGGGATA2854.4074113E-99.47235910
ACGGGTT3203.4197E-109.3746719
CGCTAGA1450.00134192059.30891228
CCCTAAA17500.09.28249551
CGTGAGC12750.09.17505823
GCGTGAG12500.09.11859722
CCCAAAG58250.08.9859151
CCCAAGT21650.08.9621221
CCGAGTA9050.08.7847132
GAGCCAC36950.08.6454926
CCCGAGT11550.08.5949031
Produced by FastQC (version 0.11.5)