FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6570752
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATT11900.013.8632510
ACGGGAT13400.013.5425329
CGTTGGA19950.013.3289891
AACGGGA17000.012.7919998
AAACGGG22600.012.54322057
GAAACGG23600.011.95317656
GGAAACG22600.011.6194875
AACGGGT5350.010.9327698
AGATCGG2751.8189894E-1110.9260751
CCCCGAT1807.7347977E-710.8501991
AAGCGGC5000.010.8041313
CCCGAAC1304.8508777E-410.4007821
GATCGGA2752.1464075E-1010.36762
CGTTCGA1756.502145E-610.3017261
CACCGCG3950.010.252480530
CGGGTAT2251.2601959E-79.99834410
CGGCTGA5300.09.9089976
CAAAGCG9700.09.7574351
ACACGTC3101.8735591E-109.67581714
GCGTGAG12500.09.5984122
Produced by FastQC (version 0.11.5)