FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6822189
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA17350.013.09575758
ACGGGAT11850.012.9091999
CGGGATT10750.012.76527210
CGTTGGA19200.012.6810531
AAACGGG21150.012.44469457
GAAACGG20850.012.3040366
GGAAACG22400.011.3594255
CGTGAGC15500.010.30470123
CACCGCG5300.010.18694930
ACAGGCG21700.09.95222718
ACCGCGC4752.7284841E-119.94567931
GCGTGAG16000.09.84207722
CGGGAAT8600.09.67858910
CCCCGTT3155.533855E-69.3039121
CCCCGAA2952.583575E-59.1704791
CGGCCTA2000.00706028858.99847139
CCCAAAG70500.08.9217051
CGAGAGT2555.6638586E-48.840821
CCGCGCC5352.8558134E-108.83027632
CAGGCGT21000.08.67709719
Produced by FastQC (version 0.11.5)