FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12522452
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA39950.012.7929981
AAACGGG39100.012.3715727
AACGGGA33100.011.9633578
TATGCCG3950.011.96149345
CGTCCGA1902.9541197E-511.8415786
GAAACGG42300.011.64841656
ACGGGAT24600.011.5240149
GGAAACG43550.010.6976315
CGGGATT22650.010.62875110
AACGGGT7300.010.4791118
CCCCGGT4551.2187229E-109.8965061
CCGGCTA12700.09.74432238
CGGCTAA12850.09.45547439
ACGCCCG7200.09.06216934
CACCGCG9200.08.55926530
CGGGAAT14350.08.4665910
CGCCCGG12050.08.40221735
GCCACCG25450.08.398313528
CCGTCCG2750.00121915178.1841345
ACCGCGC8900.08.08939731
Produced by FastQC (version 0.11.5)