FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9005800
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8950.028.40666242
CGTATGC9250.027.2421344
TCTCGTA9850.026.26796341
GATCGGA10000.025.2269841
TCGTATG10900.023.73756443
CATCTCG11200.022.09731139
ATCGGAA11650.021.8261742
ACACGTC12450.020.78228413
ATCTCGT12150.020.73989540
TCGGAAG12600.020.5377253
CGTCTGA12650.020.45370916
ACGTCTG13650.019.12009815
CACGTCT15250.017.11405614
GCACACG15850.016.60824811
GTATGCC16250.016.06088345
CGGAAGA16400.016.0534024
CGTTGGA25400.015.6959221
ACGGGAT16400.015.3653129
CGGGATT14800.014.746110
CACACGT18250.014.67063312
Produced by FastQC (version 0.11.5)