FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10662885
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA5850.021.52801341
CTCGTAT6600.020.1038842
TCGTATG7450.018.11201743
CGTATGC7200.017.8038644
GATCGGA8300.015.5032711
ATCTCGT8750.014.65003340
CGTTGGA29650.014.3900961
ATCGGAA9600.013.8678482
ACGGGAT20300.012.9613869
AAACGGG34550.012.8227097
AACGGGA28250.012.8164878
GAAACGG35450.012.7540266
ACACGTC10100.012.69156313
CGTCTGA11000.012.06204516
CGGGATT18850.012.04957310
GGAAACG36400.011.8402355
TCGGAAG11250.011.2321743
CGCGCTT1450.00666407110.8982461
CGGTATG2006.0517044E-410.1540863
CGGAAGA12550.010.0686824
Produced by FastQC (version 0.11.5)