FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7908639
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA25500.012.7381421
AAACGGG27150.012.6786397
AACGGGT6950.012.6249858
GAAACGG28000.012.4547696
AACGGGA21550.012.006098
CGGGATA3102.923116E-911.61184110
GGAAACG28500.011.5257725
ACGGGAT16700.011.0471039
CCCGATT3103.486275E-810.9147391
ACGGGTT6200.010.8863079
GATACGC1450.006834876710.86114414
TGTCGCA1450.006835519310.86100721
GCGTGAG18000.010.49897322
CGGGATT15100.010.13152310
CGGGAAT8500.010.05790310
CGTGAGC20300.09.86356723
ATAGGCG3704.7875983E-79.12084518
CCCGTAA4501.0153599E-89.022851
GCCACCG18350.08.82747528
GATTTCG12100.08.7392575
Produced by FastQC (version 0.11.5)