FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6295002
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT9200.012.5499449
CGTTGGA15250.011.7226931
AAACGGG16250.011.2575887
AACGGGA12900.011.1588748
CGGGATT9100.010.71052710
GAAACGG17450.010.5728846
GGAAACG17250.010.4482175
ACAGGCG16550.010.14749718
GCCACCG12200.09.95560228
GCGTGAG12350.09.7131922
CGTGAGC13150.09.35033123
GATCGGA1952.3488394E-59.2447871
ATCGGAA2301.7865077E-69.1424082
CGTCTAG1500.00180004139.0136671
GAGCCAC28650.09.00214826
CCGCATA1350.00771004338.9023871
CCCAAAG49000.08.5844451
CCCAAGT19100.08.4945551
AGGCGTG16000.08.4345420
CCCTAAA12950.08.3524321
Produced by FastQC (version 0.11.5)