FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4840962
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2800.019.2876145
GATCGGA3100.018.1495691
CGTTTCG3150.015.71404433
CTCGTAT3500.014.78716743
TCTCGTA4000.014.06388243
TCGGAAT3850.014.02575937
GTTTCGG3700.013.37817334
ACGGGAT8700.013.1890439
CGTTGGA12700.013.1134211
ATCTCGT4500.013.00127742
AACGGGA11350.012.6866368
AAACGGG14100.012.1277257
TTCGGAA4400.011.76118335
TCGTATG4600.011.74028444
ACCGCGC4250.011.6466431
GAAACGG15050.011.3626566
AGTCACG5350.011.35461428
CCCCGAG3602.2191671E-1011.2527331
CGCGCCC3401.033186E-911.24982733
GGAAACG14850.011.2126445
Produced by FastQC (version 0.11.5)