FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6012570
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4900.022.0330444
TCTCGTA5350.021.02044341
CTCGTAT5300.020.3742
TCGTATG5550.019.85769343
ATCTCGT6200.018.13830840
TCACGAT6450.017.08656735
ACGATCT7000.015.7440537
CACGATC7400.014.89302236
CGTCTGA8050.014.52867116
GATCGGA7200.014.4131991
ATCGGAA7650.014.1551622
CATCACG7700.014.02067733
ACACGTC8250.013.90383713
CGTTGGA15700.013.6508281
CGGGATT9050.013.42034210
GCGTGAG11750.013.39919222
ACGGGAT8900.013.3938169
AAACGGG15050.013.3006227
TCGGAAG8100.013.0901393
AACGGGA12650.012.9792858
Produced by FastQC (version 0.11.5)