FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6912189
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC172260.2492119356111356TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA20950.036.29940841
CTCGTAT21000.036.21298242
CGTATGC21350.035.72496444
TCGTATG21600.035.31122643
ATCTCGT22800.033.452540
AATCTCG23850.032.07385639
GTATGCC25850.029.7676845
CGTCTGA33000.023.59022916
GATCGGA33050.023.4594571
ACACGTC33600.023.10201513
TCGGAAG34100.022.9625433
ATCGGAA34050.022.865262
ACGTCTG35050.022.2746815
CACGTCT35500.021.99232514
CGGAAGA35300.021.9907254
GCACACG37100.020.98307211
GTAATCT36800.020.97025937
CACACGT38000.020.54531312
GTCACCT39750.019.5840629
ACCTTGT40550.019.25317632
Produced by FastQC (version 0.11.5)