FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8079236
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG5100.024.2650235
CCCGATC6150.020.85315538
GTCCCGA6200.020.32284236
CTCGTAT6350.020.19473644
TCTCGTA6500.020.07482143
TCCCGAT6250.019.80025537
CCGATCT6500.019.73004739
TCGTATG7450.017.51494445
ATCTCGT8450.015.70851242
ACCCGTC8350.015.628365532
CCGTCCC8450.014.91143534
CCCGTCC9200.013.94039733
CACCCGT10000.013.49967431
ACGGGAT16700.012.6635819
TCACCCG10550.012.58224530
CGTTGGA23550.012.3532131
AAACGGG24250.012.3395257
AACGGGA21400.012.30034358
CGGGATT15400.012.1255810
GAAACGG24050.011.7886786
Produced by FastQC (version 0.11.5)