FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3932738
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6500.035.3288345
CGTCACG1650.029.99162727
GATCGGA5550.027.2258871
CGGCTAC10850.026.12339832
CTCGTAT10850.025.29408541
TCTCGTA10850.025.29376440
ACGGCTA10900.024.97136331
CGTATGC10900.024.14647743
ATCTCGT11950.023.15341639
TCGTATG11250.022.79537442
GTCACGG11950.022.77606828
CATCTCG12350.022.58651238
TCACGGC12250.022.40190929
ACACGTC12950.021.53811512
ATCGGAA13100.021.5198351
CGTCTGA13300.021.14071515
AGTCACG10650.020.90965527
ACGTCTG13950.020.31690814
TCGGAAG13800.020.0916652
CACGTCT14550.019.9426313
Produced by FastQC (version 0.11.5)