FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6011931
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT10050.013.1303929
CGGGATT9350.012.66999910
CGTTGGA14950.012.6595141
AACGGGA13350.012.5804998
AAACGGG16450.012.2151837
GAAACGG16250.012.1840036
GGAAACG16400.011.1705215
GCGTGAG10650.010.70101722
CGTTAGT1304.8589043E-410.3988871
CCCAAGT16550.010.0742481
ACAGGCG14350.09.92733718
CGTGAGC11450.09.29852323
GAGCCAC29950.09.21259826
CCCAAAG45450.09.1544771
GCTAGAC8050.09.12769829
CCGAGTA7300.08.8453282
CCCGAGT9900.08.7999551
TACAGGC35500.08.74385717
CGGGAAT5350.08.68898410
CCCTAAT7250.08.4944161
Produced by FastQC (version 0.11.5)