FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8351991
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA25300.014.1812671
AAACGGG26150.012.9040127
AACGGGA22400.012.4533638
GAAACGG27800.012.2188286
ACGGGAT18050.011.8414159
CGGGATT16200.011.52765810
CCCCGAT2757.781637E-811.4877191
GGAAACG28800.011.4040155
CCCGATA2004.8399517E-511.2825811
CCCGGAT3154.387948E-810.7453151
CCCAACG2902.0885127E-59.3373081
CGGGAAT9200.08.804255510
CCGGATT2851.8196132E-48.6841732
CGGGATA3704.6843925E-68.51342510
AACGGGT5352.6502676E-98.4098698
GATTTCG10050.08.2819595
CCCCGAA3552.4739958E-58.2632991
CGCGCCC4406.5310996E-78.18183133
GTTGGAA49000.08.0356792
CACCGCG7507.2759576E-127.799685530
Produced by FastQC (version 0.11.5)