FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7503458
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7400.026.45354545
TATCTCG7650.024.70419940
CTCGTAT7600.024.57315343
TCTCGTA8250.023.18255642
ATCTCGT8600.022.23685641
GTCACGA8400.022.2319628
TCGTATG8950.021.1179944
TCGGAAG9600.019.7020452
ACACGTC9900.019.31712312
ATCGGAA9950.019.0090071
CGTCTGA10050.018.80481315
ACGAGTG10050.018.58181431
AGTCACG10250.018.43887527
CACGAGT10500.017.99985330
ACGTCTG11650.016.02916314
CGGAAGA12000.015.7514483
GCACACG12700.015.58969810
CACGTCT12800.014.764914513
CACACGT14700.013.92780911
GATCGGA4450.013.6617711
Produced by FastQC (version 0.11.5)