FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6192489
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1802.0008883E-1114.2067381
CGTTGGA16700.013.3310161
ACGGGAT10600.013.0162699
AACGGGA14800.012.8690528
AAACGGG17450.012.6335797
GGAAACG17400.012.4158255
CGGGATT10200.012.203444510
GAAACGG18150.011.9854336
ATCGGAA2401.8189894E-1111.8783832
GTCACGT2452.5465852E-1111.63145729
GGCGTAA1100.001436227710.90726521
ACACGTC2402.6684575E-910.62287813
TCGGAAG2701.546141E-1010.5585623
CCACGAA2352.077104E-810.2416541
CGGCTGA4300.010.1179346
CGCTAAA1200.00288857310.0282861
AAGCGGC4350.010.0028493
CCCCGAT1851.240408E-59.7572521
CCCCGAA1250.0040490459.6271541
CCCAAAG48400.09.6035351
Produced by FastQC (version 0.11.5)