FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6924974
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA21900.013.7222121
AAACGGG23600.011.7206287
AACGGGA18450.011.5793738
GAAACGG24250.011.4076216
ACGGGAT14450.011.2052749
GGAAACG24950.011.127345
CGGGATT13600.010.91346810
AACGGGT5250.010.7087448
ACGGGTT5050.010.2422259
ACCGGTA2000.00707837758.99566933
AACCGGT2300.00223855748.80004732
CGGGAAT7700.08.46964310
ACCGCGC5352.6666385E-98.40710631
CCCGAAC2550.00544767737.97478341
GTTGGAA42550.07.95698452
GCGTGAG16450.07.929256422
CGTATAA6307.694325E-107.8903941
ACAGGCG24000.07.87092718
CGTGAGC17450.07.861488323
CGGGTTT5503.8655344E-87.76870710
Produced by FastQC (version 0.11.5)