FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10193356
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA33050.015.1577561
ACGGGAT22000.014.9278299
AAACGGG36650.014.1773797
AACGGGA29600.013.9827498
CGGGATT20500.013.38666610
GAAACGG38350.013.3143036
GGAAACG39200.012.8202865
AACGGGT8300.012.1955378
CGGGATA5500.011.86046710
ACCGCGC9200.010.75794231
ACGGGTT8000.09.5599459
CACCGCG10150.09.52942730
CCGCGCC10200.09.26218432
CGCGCCC7650.09.11503233
CCGGCTA13100.09.10044338
GCCACCG27050.09.06429928
ACTGCGC10800.08.95589531
GTTGGAA62000.08.8331352
GCGTGAG27300.08.73414422
CGCCCGG14500.08.68716335
Produced by FastQC (version 0.11.5)