FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10047468
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5650.014.73412544
ACGGGAT21450.014.2651459
CGTTGGA33500.014.2465131
AACGGGA29200.013.869368
AAACGGG36600.013.7700617
GAAACGG36350.013.680036
TCGTATG5600.013.66035445
CGGGATT19850.012.92144610
TCTCGTA6300.012.85680543
AACGGGT7750.012.1931078
ATCTCGT7250.012.1031942
GGAAACG40600.012.027765
ACGGGTT7300.09.8626079
CCGGCTA9850.09.82207738
CGCCCGG10200.09.48504435
CGGGATA5302.3646862E-119.33928310
CGGCTAA9500.09.23659939
ACGCCCG4956.1299943E-109.09067134
ACAGGCG35750.09.06260918
CGGGAAT12400.08.89080610
Produced by FastQC (version 0.11.5)