FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6162693
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4300.023.6082345
GATCGGA5550.016.6797161
TATCTCG6350.014.87489141
TCTCGTA6250.014.752742
CTCGTAT6350.014.16621843
ATCTCGT6600.013.629951541
ACGAGTG7750.013.05953831
CGTTGGA18000.012.9199991
TCGTATG7500.012.893620544
GTCACGA7550.012.214581528
AAACGGG19500.011.9933857
AACGGGA14050.011.8440018
CACGAGT8250.011.72280430
GAAACGG20250.011.6625066
GGAAACG20800.011.4997715
AACGGGT5100.011.4642648
CGTCTGA8800.010.98834615
ATCGGAA8500.010.8845792
ACGGGAT10550.010.6576289
AGTCACG8750.010.53943827
Produced by FastQC (version 0.11.5)