FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8298330
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA24150.013.6112081
ACGGGAT16100.012.5660649
AACGGGA22600.012.0353738
AAACGGG27200.011.7355137
GAAACGG27600.011.5667566
GGAAACG28200.011.4089325
CGGGATT14650.011.35472810
CCCCGAT3358.740244E-910.827321
CGATAGC2005.79817E-410.2013651
CCCGGAT3053.4199857E-69.6624951
GCGTGAG20150.09.48257922
GCCACCG19800.09.42313728
ACCGCGC6650.09.1269331
CGTGAGC21000.08.99171523
CCCGAAT9300.08.7753681
CACCGCG6950.08.73296330
CACGATT3653.5438243E-68.6914242
ACCGTGC7900.08.5364431
CCACCGC15000.08.39226929
CCGGCTA8950.08.28915338
Produced by FastQC (version 0.11.5)