FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6553319
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5750.024.2603344
TCTCGTA6100.022.49967243
TATCTCG6050.022.31372641
GATCGGA6200.022.149451
GTCACGA6450.021.62709629
TCGTATG6700.020.82043545
ATCTCGT7100.019.6477542
TCGGAAG7350.019.594993
ACGAGTG7400.018.54688532
ACACGTC7550.018.4762713
ATCGGAA7650.018.2382282
CACGAGT7900.017.37303231
AGTCACG8150.017.11579328
CGGAAGA9100.015.8267224
CGTCTGA9050.015.66251716
CACGTCT9850.014.61885214
ACGTCTG9900.014.31775515
TCACGAG11150.013.9233530
GCACACG11000.012.88578211
CGTTGGA17950.012.1655591
Produced by FastQC (version 0.11.5)