FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11206739
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5250.020.99930245
CTCGTAT6400.018.28162241
TCTCGTA6300.017.500240
TCGTATG7850.014.61813242
CGTATGC7900.014.24022443
ACACGTC9200.013.4505212
ATCGGAA8900.013.4031561
ATCTCGT8800.013.04021339
CGTTGGA31400.012.5438151
AAACGGG32300.012.3289277
AACGGGA25900.011.9885548
GAAACGG33950.011.663466
TCGGAAG10350.011.5220252
ACGGGAT19750.011.3922299
CGTCTGA10100.011.35999515
GGAAACG35000.010.80036455
ACGTCTG11500.010.76036814
CGGGATT18400.010.51595410
AACGGGT8250.010.0910238
ACCGCGC8600.09.94299331
Produced by FastQC (version 0.11.5)