FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9528959
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT7750.025.83695444
TCTCGTA7900.025.06014343
TCGTATG9000.022.01479345
AACGATC9200.022.00660138
ATCTCGT9500.021.31221442
GATCGGA10450.018.5427341
GTCACGT11350.017.6397529
AGTCACG11400.017.36505328
AAACGAT11650.017.18550937
ACGATCT12600.016.42538639
ATCGGAA12350.015.8723642
CGTCTGA13350.015.839375516
ACACGTC12700.015.76441113
TCGGAAG13500.015.354453
TGAAACG13500.014.830335
CGTGAAA14350.014.26560133
CACGTGA14550.013.91489931
ACGTCTG15200.013.76356215
CGTTGGA27250.013.7256581
GAAACGA14950.013.54238136
Produced by FastQC (version 0.11.5)