FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9107559
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA25250.014.8137281
ACGGGAT16200.014.3040779
AACGGGA21250.014.18676958
AAACGGG26550.013.6426587
CGGGATT14100.013.24331210
GAAACGG27250.013.1270846
GGAAACG27800.012.4704775
GCCACCG18650.010.73615628
CACCGCG7150.010.38354230
CCCCGAT3501.8999344E-810.3007691
CGCCCGG8300.010.02908435
CCCGAAC2109.5936196E-49.6569711
CCACCGC14250.09.63058329
ACAGGCG28200.09.57347918
ACCGCGC6650.09.47270631
CGGGAAT8450.09.31856310
CCGCACC6600.09.20359432
CGTGAGC20950.09.02054723
GCGTGAG21550.08.9781922
GCCCGGC18050.08.97414236
Produced by FastQC (version 0.11.5)