FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070614

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070614
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4749717
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGT3000.014.2489298
AAACGGG14250.013.5786437
CGTTGGA14150.013.5194411
AACGGGA11850.013.1002828
GAAACGG14900.012.9862856
CGGGATT8300.011.92681510
ACGGGAT9100.011.8672429
GGAAACG15700.011.6082645
GCGTGAG12650.010.31543122
CGTGAGC12900.010.28992623
GCCACCG11100.09.72899928
CGGGAAT4904.9112714E-119.64213310
ACGGGTT3603.2177377E-79.3742969
ACCGCAC3401.4924699E-69.26410831
CCGCACC3901.0356598E-79.23017432
ACCGCGC2952.6453974E-59.15195131
CGTGCCC4304.8572474E-88.89543133
CGCACCC3053.913151E-58.8525433
CGTGTAA2300.00222309358.8066531
ACGGGAA5403.4197E-108.7493449
Produced by FastQC (version 0.11.5)