FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7281162
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA24250.014.9750411
ACGGGAT15900.013.5829219
AACGGGA20850.013.2713518
AAACGGG25300.012.8934627
CGGGATT15400.012.563110
GAAACGG26150.012.3936946
GGAAACG26900.012.0478155
CGCGCCT2402.269786E-611.24851133
CGTGAGC18250.010.72622923
GCGTGAG18250.010.72622922
GCCACCG15900.010.32903528
ACAGGCG26850.010.13971618
CCGGCTA9800.09.87141438
CCCCGTT3257.799699E-79.7162481
ACCGTGC7250.09.30930431
CAAAGCG13500.09.1893081
CGGCTAA10050.09.17814339
CGTGCCC5901.8189894E-129.15133133
ACCGCAC5007.403287E-108.99899531
CGGGAAT8300.08.94447710
Produced by FastQC (version 0.11.5)