FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070620

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070620
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12060901
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA31800.013.4113871
AAACGGG33850.012.2933147
AACGGGA27300.012.2766918
ACGGGAT20950.012.2399199
CGGGATT19400.011.36271410
GAAACGG36550.011.2005646
GGAAACG37100.010.9343845
CCCCGAT4101.8371793E-1010.457041
GCGTGAG30650.09.61447422
CACCGCG9450.09.28300830
ACAGGCG41750.09.10546918
CGCGCCC7800.08.93988633
CGTGAGC32100.08.82942123
ACCGCGC8950.08.79631831
GCCACCG26800.08.56114928
AGGCGTG38500.08.47208320
CGGGATA4001.475808E-68.43507610
CCGGCTA11550.08.37435538
GATTTCG13650.08.2553045
CAGGCGT37100.08.24608719
Produced by FastQC (version 0.11.5)