FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7896889
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT4250.016.94050442
CGTATGC4700.016.2759544
TCTCGTA4950.015.9084641
TCGTATG4850.015.7725743
CGCGCCC5750.012.9125333
GCGCCCG6300.012.85663334
ACCGCGC6700.012.76068931
CGCCCGG8500.012.70537935
CACCGCG7400.012.46572230
ACGGGAT12900.011.860079
CCCGGAC3052.230081E-911.8135811
GCGTGAG19700.011.420938522
AACGGGA18200.011.3732468
CGTGAGC19550.011.27839723
AAACGGG21150.011.1698417
CGGGATT12900.011.16241910
ATCTCGT6550.010.99193140
GCCACCG18500.010.82396428
CGTTGGA19500.010.5091661
ACAGGCG27600.010.43436818
Produced by FastQC (version 0.11.5)