FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5917249
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA15850.013.08566951
CCGTACG1607.8205456E-512.65835433
CGTACGT1651.04269064E-412.27476734
AAACGGG15600.011.680297
AACGGGA13350.011.1213088
ACGGGAT9150.010.5715969
GATTTCG7050.010.5298395
GAAACGG18600.010.522036
CGGGATT8500.010.32140610
CCCGATT2201.2856055E-410.2474231
GGAAACG18850.010.1438885
CTCGTAT2952.4545934E-69.91665244
AACGGGT3653.736568E-89.8609868
GTACGTA2302.0504369E-49.78415235
CCTTCGG2403.0882566E-49.3934711
CCCGTAA2050.0084939158.7977891
CGGGAAT5902.3646862E-118.76938310
CGGGTTT3351.2399589E-58.729547510
CCTACGG2851.78088E-48.7013211
ATTTCGT9700.08.5807096
Produced by FastQC (version 0.11.5)