FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4942013
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATT9200.014.667747510
CGTTGGA16800.014.6378411
CTCGTAT2154.1014573E-813.59934842
ACGGGAT11500.013.4948739
GAAACGG18350.013.4832986
AAACGGG18850.013.1249887
AACGGGA15500.012.9144488
GGAAACG19900.012.5820215
GATCGGA2054.690728E-612.1059441
TCTCGTA2005.0025796E-511.24584141
CGTATGC2656.23113E-711.03343344
TCGTATG3103.6381607E-810.88285243
AACGGGT4051.5643309E-1010.5515478
ATCGGAA3053.2557182E-710.3559052
ACGGGTT3552.4356268E-810.1369939
TCGGAAG3053.6329693E-69.6165883
GCCACCG9700.09.50679328
CGGGAAT5950.09.44980910
CCGCACC2403.162905E-49.3710632
GTTGGAA30950.09.2576952
Produced by FastQC (version 0.11.5)