FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11065582
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG3450.016.95559532
CGTCCGC3750.015.59914833
AACGGGA25700.013.8319298
GTCCGCA4750.013.26243334
ACGGGAT19850.013.261249
AAACGGG32250.013.1855657
CGTTGGA28900.013.1645141
GGAAACG33250.012.7914925
GAAACGG32600.012.6996116
CTCGTAT4800.012.18699944
CGGGATT19050.012.16479910
TCCGCAC6050.011.15641535
TCTCGTA5600.010.84742543
TCGTATG6850.010.510612545
CCCCGAT3755.16593E-910.2054831
CCCGGTA3653.695459E-89.8683171
CGGGAAT11000.09.61319810
GATCGGA5403.45608E-119.1715961
ATCTCGT7200.09.06184142
CCGGTAA3401.4879826E-58.6044152
Produced by FastQC (version 0.11.5)