FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8656562
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATG236110.2727526239631854TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA29600.038.3855143
CTCGTAT29500.038.3628744
TCGTATG29800.037.90160445
TATCTCG30300.037.20169441
ATCTCGT30450.037.01843642
GATCGGA37850.030.6679551
ATCGGAA39200.029.6749272
ACACGTC39000.029.65317313
CGTCTGA38900.029.44037616
TCGGAAG40250.028.8999633
ACGTCTG40700.028.24890715
CGGAAGA41600.027.7998494
CACGTCT42850.026.93637814
GCACACG44650.025.9512611
CACACGT44950.025.77805712
GTCACAT53950.021.47785829
CAGTCAC54950.021.12794127
CCAGTCA57200.020.29685826
AGAGCAC57950.020.2657228
AGCACAC59450.020.17195510
Produced by FastQC (version 0.11.5)