FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006070649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006070649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4713737
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA14450.017.5232521
AAACGGG15700.016.0410047
GAAACGG15950.015.5091026
AACGGGA13750.015.3714668
GGAAACG16650.014.5218185
ACGGGAT10100.014.4704539
CGGGATT10500.013.70505610
CCCCGAT1902.8105493E-511.8990121
CGGGAAT6650.011.83413510
ACGGGAA6650.010.8197819
AACGGGT3451.569424E-810.427768
GTTGGAA27900.09.5570682
ACGGGTT2701.01811005E-49.1604989
GCTAGAC9850.08.90263529
ACCGCGC3258.288963E-58.30289531
ACCACGC8550.08.15318331
CCGAGTA8450.08.0225312
CACCGCG3401.4029181E-47.935832530
TCGATTT4001.2574872E-57.9088783
CCACGAA2950.0023282437.66377071
Produced by FastQC (version 0.11.5)